HS-LS3-2

← HS-LS3-1

HS-LS3-3 →

Make and defend a claim based on evidence that inheritable genetic variations may result from: (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, (3) mutations caused by environmental factors and/or (4) genetic engineering.

Clarification statement: Emphasis is on using data to support arguments for the way variation occurs including the relevant processes in meiosis and advances in biotechnology.

Assessment boundary: Assessment does not include recalling the specific details of the phases of meiosis or the biochemical mechanisms of the specific phases in the process.

Regents Exam Questions

Released New York State Regents exam questions that assess HS-LS3-2 are listed here by exam and question cluster. Each link opens the question with its answer key.

Exam	Cluster	Question
August 2026 Biology Regents	Human Variations in Eye Color	Question 9
	Humongous Fungus	Question 15
	Frog Development	Question 27
June 2026 Biology Regents	Tailless Humans	Question 22
	Caffeine’s Effect on the Body	Question 40
January 2026 Biology Regents	Two Kinds of Twins	Question 3
	Solving the Problem of Cat Allergies	Question 17
August 2025 Biology Regents	Keratin – A Very Versatile Protein	Question 27
June 2025 Biology Regents	Organisms of the Tibetan Plateau	Question 18
	Nature or Nurture?	Question 46
Biology sample clusters (Spring 2024)	Prostaglandins	Question 3

Vocabulary

Terms from the Biology Glossary that come from this standard or from the released exam questions that assess it.

From this standard

• argument
• biotechnology
• cell
• chromosome
• claim
• correlation (versus cause)
• DNA replication
• empirical evidence
• environmental factor
• gene
• genetic combination
• genetic engineering
• genetic variation
• genetically modified
• inheritable variation
• meiosis
• mutation
• population
• probability
• replication error
• sex cell
• sexual reproduction
• trait
• variation

From released exam questions

• allele – June 2026, June 2025
• allergy – Jan 2026
• blood – June 2025
• body cell – June 2026, Aug 2025
• coloration – Aug 2026
• CRISPR-Cas9 (gene editing) – Jan 2026
• cross (genetic cross) – June 2026
• differentiation – Aug 2026
• DNA – Aug 2026, June 2026, Jan 2026, Aug 2025, June 2025, Sample 2024
• DNA sequence – Jan 2026, Sample 2024
• egg (egg cell) – Aug 2026, Jan 2026
• enzyme – Sample 2024
• fraternal twins – Jan 2026
• gamete – June 2026, June 2025
• gene expression – June 2025
• gene therapy – Aug 2025
• generation – June 2026, Aug 2025, June 2025
• genetic technology – Jan 2026
• genotype – June 2026
• hybrid – June 2026
• inflammatory response – Sample 2024
• insertion (mutation) – June 2026
• keratinocyte – Aug 2025
• methylation (and demethylation) – June 2025
• mitosis – Aug 2026, June 2026, Aug 2025, June 2025
• neuron (nerve cell) – June 2026
• nucleic acid – June 2025
• oxygen – June 2025
• parental generation (P) and F₁, F₂ generations – June 2026
• phenotype – June 2026
• pigment – Aug 2026
• prostaglandin receptor – Sample 2024
• protein – Jan 2026, Sample 2024
• recombination – Aug 2026
• reproduction – Sample 2024
• reproductive cell – Jan 2026
• salivary gland – Jan 2026
• skin – Aug 2026, Jan 2026
• skin cell – Aug 2025
• sperm – Aug 2026, Jan 2026
• spore – Aug 2026
• sugar – Sample 2024
• ultraviolet radiation – Aug 2026
• variant (gene variant) – June 2026
• zygote – Aug 2026

Implied by this standard

• crossing over – implied by "chromosomes can sometimes swap sections during the process of meiosis"

Performance Level Descriptions

Every Regents exam question on HS-LS3-2 is written to match one of the performance level descriptions (PLDs) below.

Level 1: Identify a claim, from those provided, based on evidence that inheritable genetic variation occurs. Aug 2026 · Q27 Jan 2026 · Q17 June 2025 · Q46

Level 2: Identify a claim based on evidence for a cause of inheritable genetic variation. Aug 2026 · Q9 June 2026 · Q40 Aug 2025 · Q27 June 2025 · Q18 June 2025 · Q3

Level 3: Make or defend a claim based on evidence that inheritable genetic variations may result from: (1) new genetic combinations through meiosis and/or (2) viable errors occurring during replication, and/or (3) mutations caused by environmental factors and/or (4) genetic engineering. Aug 2026 · Q15 June 2026 · Q22 Jan 2026 · Q3

Level 4: Make and defend a claim based on evidence that inheritable genetic variations may result from: (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, (3) mutations caused by environmental factors and/or (4) genetic engineering.

Level 5: Make and defend a claim based on evidence regarding the causes of inheritable genetic variations, which may result from: (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, (3) mutations caused by environmental factors and/or (4) genetic engineering, and the variation's effect.

Notice: PLD levels!

Resources

Examples and discussion of resources for the learning, teaching, and assessment of HS-LS3-2.

This section could be expanded upon. You can help out by adding to this section.

NGSS Dimensions

Performance expectation HS-LS3-2 was developed using the following elements from the NRC document A Framework for K-12 Science Education:

Science and Engineering Practices

• Engaging in Argument from Evidence
  • Make and defend a claim based on evidence about the natural world that reflects scientific knowledge, and student-generated evidence.

Disciplinary Core Ideas

• LS3.B: Variation of Traits
  • In sexual reproduction, chromosomes can sometimes swap sections during the process of meiosis (cell division), thereby creating new genetic combinations and thus more genetic variation. Although DNA replication is tightly regulated and remarkably accurate, errors do occur and result in mutations, which are also a source of genetic variation.
  • (NYSED) Environmental factors can cause mutations in genes. Only mutations in sex cells can be inherited.
  • (NYSED) Advances in biotechnology have allowed organisms to be modified genetically.
  • Environmental factors also affect expression of traits, and hence affect the probability of occurrences of traits in a population. Thus the variation and distribution of traits observed depends on both genetic and environmental factors.

Crosscutting Concepts

• Cause and Effect
  • Empirical evidence is required to differentiate between cause and correlation to make claims about specific causes and effects.

Page contributors: Conrad Richman, Caroline Leonard

Biology | HS. Inheritance and Variation of Traits