HS-LS4-2

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Construct an explanation based on evidence that the process of evolution primarily results from four factors: (1) the potential for a species to increase in number, (2) the heritable genetic variation of individuals in a species due to mutation and sexual reproduction, (3) competition for limited resources, and (4) the proliferation of those organisms that are better able to survive and reproduce in the environment.

Clarification statement: Emphasis is on using evidence to explain the influence each of the four factors has on number of organisms, behaviors, morphology, or physiology in terms of ability to compete for limited resources and subsequent survival of individuals and adaptation of species. Examples of evidence could include mathematical models such as simple distribution graphs and proportional reasoning.

Assessment boundary: Assessment does not include other mechanisms of evolution, such as genetic drift, gene flow through migration, and co-evolution.

Regents Exam Questions

Released New York State Regents exam questions that assess HS-LS4-2 are listed here by exam and question cluster. Each link opens the question with its answer key.

Exam	Cluster	Question
August 2026 Biology Regents	Star of the Tide Pool	Question 1
June 2026 Biology Regents	A Seasonal Strategy for Survival	Question 5
	Green and Black Frogs	Question 11
	Buzzy Bees	Question 29
	Australian Adaptations	Question 44
January 2026 Biology Regents	Honeybee Behavior and Threats	Question 31
	Baboon Evolution	Question 34
	Zebra Coat Patterns	Question 43
August 2025 Biology Regents	Strategies for Survival in Different Animal Species	Question 6
	Strategies for Survival in Different Animal Species	Question 9
	Keratin – A Very Versatile Protein	Question 28
	Evolutionary Relationships	Question 34
June 2025 Biology Regents	Drinking Water Is Just The Beginning!	Question 7
	Heads or Tails?	Question 12
	Limb Evolution	Question 30
	Limb Evolution	Question 32
Biology sample clusters (Spring 2024)	Dinosaurs-Evolution	Question 1

Vocabulary

Terms from the Biology Glossary that come from this standard or from the released exam questions that assess it.

From this standard

• adaptation
• behavior
• claim
• competition
• correlation (versus cause)
• empirical evidence
• environmental factor
• evolution
• gene
• genetic information
• genetic variation
• investigation
• limited resources
• migration
• morphology
• mutation
• natural selection
• physiology
• population
• proliferation
• reproduction
• reproduction potential
• resources
• sexual reproduction
• species
• survival
• trait
• variation

From released exam questions

• aquatic – Aug 2025
• asexual reproduction – June 2025
• asteroid impact – Sample 2024
• atmosphere – Sample 2024
• Australia – June 2026
• average – June 2026, Sample 2024
• blood – Aug 2026
• blood vessel – June 2025
• bone – June 2025
• Bowman’s capsule – June 2025
• camouflage – June 2026
• carbon – Sample 2024
• climate – June 2026
• colony – June 2026
• coloration – June 2026
• digestive system – June 2026
• disease – Jan 2026
• DNA – Aug 2025
• DNA sequence – Aug 2025
• egg (egg cell) – June 2026, June 2025
• frequency – June 2026
• fungus – June 2026
• generation – Aug 2025, June 2025
• genetic material – June 2026
• genome – Aug 2025
• gland – Aug 2026
• habitat – June 2025
• herbivore – Sample 2024
• herding – Aug 2025
• hormone – June 2026
• host – June 2025
• hybrid – Aug 2026
• hypothesis – Jan 2026
• immune response – June 2026
• infection – June 2026
• interbreeding – Aug 2026
• invertebrate – Aug 2026
• K-T extinction – Sample 2024
• kidney – June 2025
• larva – June 2025
• life cycle – June 2025
• mammal – June 2026, Aug 2025, June 2025, Sample 2024
• mating – June 2026
• metabolism – June 2026
• nephron – June 2025
• ocean – June 2025
• parasite – June 2025
• parental care – Aug 2025
• pheromone – June 2026
• photosynthesis – Sample 2024
• pigment – June 2026
• predation – June 2025
• predator – Aug 2026, June 2026, Aug 2025, June 2025
• prey – Aug 2026, June 2025
• producer – Aug 2026
• rainforest – June 2026, Jan 2026
• reproductive cycle – Aug 2025
• resistance (to a pesticide or treatment) – Jan 2026
• salinity – Aug 2026
• skin – Jan 2026
• stinger – June 2026
• stomach – Aug 2026
• structure and function – Aug 2025, June 2025
• sulfur – Sample 2024
• terrestrial – June 2025
• tide pool – Aug 2026
• tubule – June 2025
• waste – June 2025
• white blood cell – June 2026

Implied by this standard

• convergent evolution – Aug 2025 Evolutionary Relationships asks why sharks (fish) and marine mammals evolved similar body shapes, without naming the idea.
• selection pressure (environmental pressure) – implied by "competition for an environment’s limited supply of the resources"

Performance Level Descriptions

Every Regents exam question on HS-LS4-2 is written to match one of the performance level descriptions (PLDs) below.

Level 1: Identify the evidence, from those provided, that supports the explanation that evolution is the result of a genetic and/or an environmental factor. June 2026 · Q11 Jan 2026 · Q34 June 2025 · Q32

Level 2: Identify the explanation based on evidence that evolution is the result of genetic and/or environmental factor(s) or justify why the given explanation is correct or incorrect. Aug 2026 · Q1 Jan 2026 · Q31 Jan 2026 · Q43 Aug 2025 · Q34 Aug 2025 · Q28 June 2025 · Q1 June 2025 · Q7 June 2025 · Q12

Level 3: Construct an explanation based on evidence that the process of evolution can involve: (1) the potential of species to increase in number, and/or (2) the heritable genetic variation of individuals in a species due to mutation and sexual reproduction, and/or (3) competition for limited resources, and/or (4) the proliferation of those organisms that are better able to survive and reproduce in the environment. June 2026 · Q5 June 2026 · Q44 June 2026 · Q29 Aug 2025 · Q6 Aug 2025 · Q9 June 2025 · Q30

Level 4: Construct an explanation based on evidence that the process of evolution primarily results from four factors: (1) the potential of species to increase in number, (2) the heritable genetic variation of individuals in a species due to mutation and sexual reproduction, (3) competition for limited resources, and (4) the proliferation of those organisms that are better able to survive and reproduce in the environment.

Level 5: Construct an explanation based on evidence obtained from a variety of reliable sources that natural selection is a process leading to biological evolution of a species, primarily caused by four factors: (1) the potential of species to increase in number, (2) the heritable genetic variation of individuals in a species due to mutation and sexual reproduction, (3) competition for limited resources, and (4) the proliferation of those organisms that are better able to survive and reproduce in the environment.

Notice: PLD levels!

Resources

Examples and discussion of resources for the learning, teaching, and assessment of HS-LS4-2.

This section could be expanded upon. You can help out by adding to this section.

NGSS Dimensions

Performance expectation HS-LS4-2 was developed using the following elements from the NRC document A Framework for K-12 Science Education:

Science and Engineering Practices

• Constructing Explanations and Designing Solutions
  • Construct an explanation based on valid and reliable evidence obtained from a variety of sources (including students’ own investigations, models, theories, simulations, peer review) and the assumption that theories and laws that describe the natural world operate today as they did in the past and will continue to do so in the future.

Disciplinary Core Ideas

• LS4.B: Natural Selection
  • Natural selection occurs only if there is both (1) variation in the genetic information between organisms in a population and (2) variation in the expression of that genetic information – that is, trait variation – that leads to differences in performance among individuals.
• LS4.C: Adaptation
  • Evolution is a consequence of the interaction of four factors: (1) the potential for a species to increase in number, (2) the genetic variation of individuals in a species due to mutation and sexual reproduction, (3) competition for an environment’s limited supply of the resources that individuals need in order to survive and reproduce, and (4) the ensuing proliferation of those organisms that are better able to survive and reproduce in that environment.

Crosscutting Concepts

• Cause and Effect
  • Empirical evidence is required to differentiate between cause and correlation and make claims about specific causes and effects.

Page contributors: Conrad Richman, Caroline Leonard

Biology | HS. Natural Selection and Evolution