August 2025 Biology Regents Exam · questions and answer key

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The August 2025 Biology Regents exam (the Regents Examination in Life Science: Biology) has 45 questions, 27 multiple choice and 18 constructed response, organized into ten question clusters. Select a cluster to see its questions with the answer key. Each question is listed with the NYSSLS performance expectation it assesses. Data on the percentage of students answering correctly is not yet available.


Trees Have Organ Systems · Questions 1–4

Question Standard % correct
Question 1 HS-LS1-2 —
Question 2 HS-LS1-3 —
Question 3 HS-LS2-2 —
Question 4 HS-ESS2-6 —



Strategies for Survival in Different Animal Species · Questions 5–10

Question Standard % correct
Question 5 HS-LS2-8 —
Question 6 HS-LS4-2 —
Question 7 HS-LS2-2 —
Question 8 HS-LS4-3 —
Question 9 HS-LS4-2 —
Question 10 HS-LS4-4 —



A Unique Relationship: Mulberries and Silk · Questions 11–14

Question Standard % correct
Question 11 HS-LS1-7 —
Question 12 HS-LS1-6 —
Question 13 HS-LS1-2 —
Question 14 HS-LS1-1 —

Ivory and Elephants · Questions 15–19

Question Standard % correct
Question 15 HS-LS2-2 —
Question 16 HS-LS3-3 —
Question 17 HS-LS4-4 —
Question 18 HS-LS2-6 —
Question 19 HS-ETS1-3 —

Carbon Cycle Supports Life on Earth · Questions 20–23

Question Standard % correct
Question 20 HS-LS2-5 —
Question 21 HS-LS2-5 —
Question 22 HS-ESS2-6 —
Question 23 HS-ESS2-6 —



Keratin – A Very Versatile Protein · Questions 24–28

Question Standard % correct
Question 24 HS-LS1-1 —
Question 25 HS-LS1-4 —
Question 26 HS-LS1-1 —
Question 27 HS-LS3-2 —
Question 28 HS-LS4-2 —



How Do Kangaroo Rats Survive in the Desert? · Questions 29–32

Question Standard % correct
Question 29 HS-LS1-2 —
Question 30 HS-LS4-4 —
Question 31 HS-LS1-2 —
Question 32 HS-LS2-1 —



Evolutionary Relationships · Questions 33–36

Question Standard % correct
Question 33 HS-LS4-1 —
Question 34 HS-LS4-2 —
Question 35 HS-LS4-1 —
Question 36 HS-LS4-1 —



The Human Journey from One Cell to 30 Trillion · Questions 37–41

Question Standard % correct
Question 37 HS-LS1-8 —
Question 38 HS-LS1-4 —
Question 39 HS-LS1-8 —
Question 40 HS-LS1-4 —
Question 41 HS-ETS1-3 —

Driving the Florida Panther to Extinction · Questions 42–45

Question Standard % correct
Question 42 HS-LS4-4 —
Question 43 HS-LS2-4 —
Question 44 HS-LS4-5 —
Question 45 HS-ETS1-3 —
Page contributors: Caroline Leonard